CGBQ

CGBQ

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  • 2025年12月10日
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    • 详细信息
    • 询价记录
    • 技术资料
    • 年限

      15 to 17 days gestation

    • 物种来源

      其他

    • 是否是肿瘤细胞

      0

    • 生长状态

      贴壁生长

    • 细胞类型

      成纤维细胞

    • 相关疾病

      正常

    • ATCC Number

      CCL-169™

    • 器官来源

      其他

    • 细胞形态

      成纤维样

    • 库存

      大量

    • 运输方式

      冻存运输

    Designations: CGBQ
    Depositors:  HG Coon
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Anser anser
    Morphology: fibroblast

    Source: Organ: sternum
    Disease: normal
    Cell Type: fibroblast
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Virus Susceptibility: Herpes simplex virus
    Sindbis virus
    Vesicular stomatitis virus
    Human poliovirus 1
    Cytogenetic Analysis: range 80 to 145
    Exact chromosome count to determine the modal number is difficult to achieve owing to the large number of microchromosomes which complicate the picture. There are 20-27 macrochromosomes.
    Age: 15 to 17 days gestation
    Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
    Medium Renewal: 2 to 3 times per week
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) glycerol
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
    recommended serum:ATCC 30-2020
    References: 21858: . . Carnegie Inst. Wash. Year Book 67: 421, 1968.
    22582: Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097

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