重组人UBE2C(带His标签)报价

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产品名称：重组人UBE2C(带His标签)
英文名称：Recombinant Human Ubiquitin Conjugating Enzyme E2 C, His
产品货号：GS4872
产品规格：10μg

分子量：Approximately 21.1kDa,a single non-glycosylated polypeptide chain containing 179 amino acids(a.a.)of human UBE2C/UBCH10 and 12 a.a.vector sequence including 6×His tag at N-terminus.
储存条件：冷冻（-20℃）

概述

Ubiquitin Conjugating Enzyme E2 C belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2C gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The UBE2C catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex,and therefore,plays an important role in the control of the cell exit from mitosis.This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle.In addition,UBCH10 bears homology to yeast PAS2,a gene that is essential for biogenesis of peroxisomes.UBCH10 is useful for in vitro ubiquitinylation reactions.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Data is not available.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of rHuUBE2C,His as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

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