BstMWI价格

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产品名称：BstMWI
英文名称：BstMWI
产品货号：GS2229
产品规格：250U

储存条件：冷冻（-20℃）

使用说明

At 37℃ activity is 20%from maximum

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sites	GCNNNNN↑NNGC
来源	Bacillus stearothermophilus MW
Assayed on	Lambda DNA
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	55℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;200 μg/ml BSA,7 mM 2-mercaptoethanol;and 50%glycerol.|Store at -20℃.|For long term storage(more than 7 days),store at -70℃.
Ligations	After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 55℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	HpyF10VI,MwoI

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