重组PreScission酶(低内毒素)

特别提示：包括重组PreScission酶(低内毒素)在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组PreScission酶(低内毒素)
英文名称：Recombinant PreScission Protease, Low Endotoxin
产品货号：GS4741
产品规格：100IU

储存条件：冷冻（-20℃）

概述

PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease.rPP is a fusion protein of glutathione S-transferase(GST)and human rhinovirus(HRV)type 14 3C protease.It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals,but also upon the super structures of the fusion protein.rPP works most effective at 4℃ and can digest substrates at room temperature as well.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE

技术规格
 

外观	Sterile colorless liquid.
来源	Escherichia coli.
内毒素	Less than 0.1 EU/μg of rPPase as determined by LAL method.
Cleavage Buffer	50mMTris-HCl,pH 7.0(at 25℃),150mMNaCl,1 mM EDTA,1 mM dithiothreitol.Chill to 5 ℃ prior to use.
Unit Definition	One unit is defined as the amount of enzyme needed to cleave 100μgof fusion protein in 16hoursto 90%completion at 5 ℃ in a buffer containing 50mMTris-HCl,pH 7.0,150mMNaCl,1 mM EDTA,and 1 mM DTT.
Recommended Conditions for Cleavage of a Fusion Protein	During cleavage reactions,it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield,purity,and extent of digestion.The amount of PreScission Protease,temperature and length of incubation required

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