重组TEV蛋白酶北京品牌

特别提示：包括重组TEV蛋白酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组TEV蛋白酶
英文名称：Recombinant Tobacco Etch Virus Protease
产品货号：GS4632
产品规格：300IU

储存条件：冷冻（-20℃）

概述

TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a(NIa)protein.It is consists of 241 a.a.with the molecular weight of 27 k Da.TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q(or S)/X’,and cleaves between Q(or S)/X’.In this form×and X’ stand for any of the amino acid residues,except that X’ cannot be P.The optimal cleavage site is ENLYFQ/G.As having the absolute specificity and wildly using conditions like broad pH range and ionic strength,the TEV protease became more versatile than EK,thrombin and other protease used in biochemical applications,especially recombinant protein production.The optimal temperature for cleavage is 30 ℃;however,the enzyme can be used at temperatures as low as 4 ℃.Following digestion,TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography.

使用说明

This material is offered by Biorab Biotechk for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Clear colorless liquid.
来源	Escherichia coli.
Recommended Conditions for Cleavage of a Fusion Protein	A number of variables can be changed to optimize the cleavage of any specific protein.The amount of rTEV,the temperature of the incubation,and the time needed for cleavage may be examined.If the protein of interest is heat-labile,then 4 ℃ incubation
Unit Definition	One unit is defined as the amount of enzyme needed to cleave 3μgof fusion protein in 1hourto 85%completion at 30℃ in a buffer containing 50mMTris-HCl,pH 8.0,0.5 mM EDTA,and 1 mM DTT.

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