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Anti-Human Ig, kappa,Gt, FITC 1MG
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文献和实验Human immunoglobulin allotypes are antigenic determinants (or “markers”) determined serologically, classically by hemagglutination inhibition, on the human immunoglobulin (IG) heavy and light chains. The allotypes have been identified
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
at 4 °C with FITC-conjugated goat anti-mouse anti-Ig antibody fragments (1/50; DAKO Glostrup, Denmark). After washings in PBS–FCS, nuclei were analysed by flow cytometry on a FACSvantage SE (BD Biosciences, San Jose, CA, USA) equipped with the pulse
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