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说明书为准
- 抗体英文名:
Anti-CD28 (human), FITC, clone CD28.2
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文献和实验实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
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