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Anti-Green Red Cone Opsin, clone 7G8
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文献和实验Anatomy of a Comparative Gene Expression Study
their presence. The reporters currently used in comparative hybridization to microarrays are fluorescent dyes (fluors), represented by the red and green circles attached to the cDNA's in the diagram. A differently-colored fluor is used for each sample
Fluorescence Activated Cell Sorting (FACS)
of liquid, they are scanned by a laser (blue light in figure 1). Some of the laser light is scattered (red cone emanating from the red cell) by the cells and this is used to count the cells. This scattered light can also be used to measure the size
This technique can be used to isolate overlapping DNA fragments starting with a previously cloned DNA fragment that maps near a gene of interest (dark red). The walk is continued until a clone containing the desired gene is identified
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