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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 适应物种:
Human, Mouse, Rat
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 宿主:
Mouse Monoclonal Antibody
- 应用范围:
WB, IP, IF
- 抗体英文名:
TAL1 / SCL LS-C182473 Antibody
- 抗体名:
TAL1 / SCL LS-C182473 Antibody
- 规格:
100ul
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
off the PBS. Add 100 µL of 1X PBS to each tube. Add 4 µg of antibody to one tube and 4 µg of preimmune serum to the other. Rotate for 4-6 h at 4°C. 31. Using the magnetic stand, aspirate the supernatants and wash the beads in each tube
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells
(including an equal amount of a normal IgG control) and immune complexes were allowed to form by slow mixing on a rotating platform at 4ºC overnight. Although antibody concentrations will vary, a guideline can be to begin within the range of 0.5�5µg/ml
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