- ￥2500
- Signalway Antibody LLC，SAB
- 美国
- 13435
- 2025年07月08日
- WB,ICC,IF,IHC,IP,FC
- Recombinant Rabbit Monoclonal Antibody
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C
- 适应物种：
Human, Mouse
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 宿主：
Recombinant Rabbit Monoclonal Antibody
- 应用范围：
WB,ICC,IF,IHC,IP,FC
- 抗体英文名：
Phospho-Creb (S133) Antibody
- 抗体名：
Phospho-Creb (S133) Antibody
- 规格：
100ul
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文献和实验antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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