- ¥1018
- Thermo Scientific™ Oxoid™
- CM0031B/ CM1176B
- 英国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
Thermo Scientific™ Oxoid™
- 规格：
500g
Brilliant Green Bile Broth 2% (Dehydrated)
相关应用：
Industrial Microbiology
Detect and confirm the presence of members of coli-aerogenes group in water, wastewater, foods, milk, and dairy products with Thermo Scientific™ Oxoid™ Brilliant Green Bile Broth 2% (Dehydrated). The medium has been formulated to select for coliform bacteria which are recognised by rapid production of gas through lactose fermentation. Bile salts and brilliant green in the medium inhibit the Gram-positive and Gram-negative bacteria respectively.
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描述
Brilliant Green Bile Broth 2% was first described by Durham and Schoenlein1 to selectively recover organisms of the coli-aerogenes group. The concentration of the components of the medium is optimized so that anaerobic lactose fermenters are inhibited without the coliforms being affected.
- Easy-to-read: coliform bacteria are easy to recognize by rapid production of gas through lactose fermentation.
- Selects for coliform organisms: bile salts and brilliant green in the medium inhibit nontarget Gram-positive and Gram-negative organisms. The medium is further optimized to prevent the growth of anaerobic lactose fermenters without coliforms being affected.
Not all products are available for sale in all territories. Please inquire.
Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
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For Laboratory Use Only
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文献和实验that you can download from the Invitrogen website. For the Two colour FISH, we recommend to use the Alexa Fluor 488 dye (FISH Tag™ DNA Green Kit, Cat. No. F32947) and the Alexa Fluor 555 dye (FISH Tag™ DNA Orange Kit, Cat. No. F32948). Note that for the Nick Translation
Site‐Specific Protein Labeling with SNAP‐Tags
Cell‐impermeable BG‐S‐S‐Alexa Fluor 488 (for probe synthesis, see Cole and Donaldson, ) TCEP (Sigma or Thermo Scientific; a 500 mM stock is prepared in water
. If IPTG induction is needed, transfer 200 µl of the saturated culture to 10 ml of L broth with antibiotic. IPTG may be added at this time, or the cells may be grown to attain exponential growth before adding the IPTG. Add 10 - 100 µl of 1 M IPTG
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