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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
Thermo Scientific™ Oxoid™
- 规格:
500g
Tetrathionate Broth Base (Dehydrated)
相关应用:
Industrial Microbiology
Selectively enrich and isolate Salmonella typhi and other salmonellae from feces and sewage with Thermo Scientific™ Oxoid™ Tetrathionate Broth Base (Dehydrated). Organisms which reduce tetrathionate, such a salmonellae, flourish in the medium while many fecal organisms are inhibited1.
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描述
Salmonella infection (salmonellosis) is a common bacterial disease that affects the intestinal tract. Salmonellabacteria typically live in animal and human intestines and are shed through feces. Humans become infected most frequently through contaminated water or food.
Tetrathionate Broth is recommended for the selective enrichment method of isolating Salmonella typhi and other salmonellae from feces, sewage, etc.
The combination of thiosulphate and tetrathionate render selectivity to the medium, suppressing commensal coliform organisms. Organisms which possess the enzyme tetrathionate reductase grow in the medium. Salmonella and Proteus species possess the enzyme; Escherichia coli and shigellae do not. Members of Proteus group reduce tetrathionate and may consequently impair the value of this medium for the isolation of salmonellae; this disadvantage of the medium is largely overcome by the addition of 40µg of novobiocin to each milliliter of the incomplete medium before the addition of iodine2,3.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
‹ 收起
For Laboratory Use Only
1. Knox R., Gell P. G. H. and Pollock M. R. (1942) J. Path. Bact. 54. 469-483.
2. Jeffries L. (1959) J. Clin. Path. 12. 568-571.
3. Buttiaux R., Catsaras M. and Verdant M. (1961) Ann. Inst. Pasteur de Lille 12. 13-18.
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文献和实验Ribonuclease A (RNase A), DNase-free
pancreas.Molecular Weight13.7 kDa monomer.ApplicationsPlasmid and genomic DNA preparation (3, 4), ..Removal of RNA from recombinant protein preparations.Ribonuclease protection assays (3).Mapping single-base mutations in DNA or RNA (5, 6).Quality Control
Denaturing Agarose Gel Electrophoresis of RNA
structure via intramolecular base pairing, and this prevents it from migrating strictly according to its size. Be sure to include a positive control RNA on the gel so that unusual results can be attributed to a problem with the gel or a problem with the RNA
Endothelial Cell Systems - Instructions For Use
and prepare for initial set up. Base your set up on the number of cells indicated on the accompanying Certificate of Analysis. (See Appendices B and C). Check the calibration on humidified incubator. Incubator should be a 5% CO2/95% air, humidified
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