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文献和实验Transmigration of Human CD34+ Cells
to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.
Polyclonal Expansion of Human CD4+CD25+ Regulatory T Cells
for the efficient in vitro expansion of human Treg cells and identified CD4+ CD25high CD45RA+ T cells as the ideal starting population for the generation of homogeneous and stable Treg cell products. Here, we provide detailed instructions for their identification
Human CD4+CD25highCD127low/neg Regulatory T Cells
CD4+CD25high CD127low/neg regulatory T cells (Tregs) play a critical role in the maintenance of peripheral tolerance and in controlling the development of autoimmune diseases. A combination of surface and intracellular markers, namely, CD
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