Bse21I内切酶优惠促销

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产品名称：Bse21I内切酶
英文名称：Bse21I
产品货号：GS2080
产品规格：500U

储存条件：冷冻（-20℃）

技术规格

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	CC↑TNAGG
来源	Bacillus species 21
Assayed on	Lambda DNA(HindIII-digest)
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM KH2PO4(pH 7.4);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 2-fold overdigestion with enzyme about 50%of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10%PEG.Of these more than 90%can be recut
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 30 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	AocI,AxyI,Bsu36I,CvnI,Eco81I,SauI

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