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Popular cell junction antibodies
| Catalog # | Name | Size |
|---|---|---|
| MA324000A488 | P-cadherin Monoclonal Antibody (12H6), Alexa Fluor 488 | 50 µL |
| MA324000A555 | P-cadherin Monoclonal Antibody (12H6), Alexa Fluor 555 | 50 µL |
| MA324000A647 | P-cadherin Monoclonal Antibody (12H6), Alexa Fluor 647 | 50 µL |
| MA339100A488 | ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488 | 50 µL |
| MA339100A555 | ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 555 | 50 µL |
| MA339100A647 | ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 647 | 50 µL |
What are cell junctions?
Cell junctions regulate small-molecule trafficking between cells, the organization of cells into tissues, and the adherence of cells to each other and the extracellular matrix. Dysfunctional junctions are implicated in several diseases, including cancer, neurodegeneration, and cardiovascular dysfunction. Understanding cell junctions is fundamental to understanding disease pathology.
Gap junctions
Figure 1—Connexin-40 and Connexin-43. Immunofluorescent staining of mouse heart atrium using mouse anticonnexin-43 antibody (red) and mouse anti-connexin-40 antibody (green). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.
Gap junctions are clusters of tightly packed channels that allow small molecules (metabolites, second messengers, and ions) to travel between adjoining cells1. This molecular exchange is essential during development and differentiation; it also creates cell proliferation, tumor suppression, cell death propagation, and survival-modulating signals2. These signals include connexins, and pannexin 1 and 2, important components of synapses3. Several kinases phosphorylate connexins and alter their functions: V-src kinase, C-src kinase, PKC, MAPK, cdc2 kinase, casein kinase I, and PKA.
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文献和实验目前市场上的 ELISA 试剂盒质量参差不齐,如何去挑选适合自己的试剂盒就显得特别重要,具体有以下几点可供参考。 特异性 ELISA 试剂盒的特异性与试剂盒的关键组分,抗体对有关。若抗体对中之一为多抗,另一个必须为单抗,建议使用双单抗。 灵敏度 灵敏度反映的是试剂盒检出被检物质的最低量的能力,用户可根据自己样本中待检指标的量选择合适的试剂盒,如果待检指标量很低,一般的试剂盒不能满足要求,可选择高敏的 ELISA 试剂盒。 重复
目前市场上的ELISA试剂盒质量参差不齐,如何去挑选适合自己的试剂盒就显得特别重要,具体有以下几点可供参考: 一、特异性 ELISA试剂盒的特异性与试剂盒的关键组分,抗体对有关,若抗体对中之一为多抗,另一个必须为单抗,建议使用双单抗。 二、灵敏度 灵敏度反映的是试剂盒检出被检物质的最低量的能力,用户可根据自己样本中待检指标的量选择合适的试剂盒,如果待检指标量很低,一般的试剂盒不能满足要求,可选择高敏的ELISA试剂盒。 三、重复
,一组使用 NaHS 处理,一组则不作处理;然后用荧光马来酰亚胺染料 Alexa Fluor 488 C5 maleimide(下文简称MAL,Invitrogen,A10254)标记半胱氨酸上的硫醇基团,使正常的半胱氨酸通过 -S- 与 MAL 连接,而硫巯基化的半胱氨酸通过二硫键 -S-S- 与 MAL 连接,如下图: 图 1. 文献 Fig. 6G,硫巯基化分析步骤 接下来加入 DTT,硫巯基化蛋白上特有的二硫键就会被解开,导致荧光 MAL 分子脱落,荧光信号减弱。所以,荧光信号发生变化就验证
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