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- 详细信息
- 文献和实验
- 技术资料
- 规格:
1000 assays
细胞活性测定试剂盒广泛应用于细胞生物学的各类实验中,例如研究生长因子、细胞活素类和培养基组分,细胞毒性因子和淋巴细胞的活性。
MTT和XTT都是检测细胞存活和生长的方法,MTT的产物为水不溶性的蓝紫色结晶甲瓒(Formazan),并沉积在细胞中,需要加有机溶剂溶解,由于在去上清的操作时会有可能带走小部分的甲瓒,故重复性略差。XTT作为线粒体脱氢酶的作用底物,被活细胞还原成水溶性的橙黄色甲臢产物,当XTT与电子偶合剂(例如PMS)联合应用时,其所产生的水溶性的甲臢产物的吸光度与活细胞的数量成正比。
XTT优点:
1. 使用方便,省去了洗涤细胞
2. 检测快速
3. 灵敏度高,甚至可以测定较低细胞密度
4. 重复性优于MTT
细胞活性测定试剂盒适用于单核细胞(Monocytes),人肝癌细胞Hep G2胚胎纤细胞 Ehd-1, 小鼠纤维细胞,恶性黑色素瘤A375,胰腺癌BXPC-3等30余种细胞类型。
产品官网链接:https://www.bioind.com/worldwide/cell-proliferation-kit/
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. 实验步骤 For staining cells prior to flow cytometric analysis of cell proliferation or cell division, the protocol below is appropriate. More information on this procedure can be found in reference 7. Our suggested initial conditions
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