L-15 Medium (Leibovitz) was originally formulated for use in carbon dioxide free systems requiring sodium bicarbonate supplement. L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue.
However, after appropriate supplementation, this complex media formulation relative to other media types such as balanced salt solutions or basal media formulations, can specifically support established cell lines such as HEp-2 cells (i.e. used for identification of antinuclear auto-antibodies or ANA) and LLC-MK2 also known as Lipid Metabolism of Monkey Kidney cells. Leibovitz L-15 Medium has also been successfully utilized for the cultivation of viruses.