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500ml
Gibco™ KnockOut™ Serum Replacement – Multi-Species (KnockOut SR – Multi-Species) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this Research Use Only product uses the same KnockOut Serum Replacement formulation that has been trusted by many for almost 20 years. For pre-clinical or clinical applications, please see Cat. No. 10828028.
With KnockOut Serum Replacement – Multi-Species, you can:
• Culture mouse, human, and other mammalian feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS
Culture mammalian PSCs with confidence
The KnockOut SR – Multi-Species formulation has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from mouse, human, and other mammalian species. For mouse and human PSCs, it has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.
Rely on improved performance
KnockOut SR – Multi-Species has a more defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing mouse and human PSCs with KnockOut SR – Multi-Species leads to less differentiation and more compact colony morphology compared to FBS.
Enjoy the peace of mind of having stable price and supply
KnockOut SR – Multi-Species is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.
规格
| Manufacturing Quality: | cGMP for medical devices, 21 CFR Part 820 and ISO 13485 |
|---|---|
| Classification: | Serum-Free |
| Product Line: | KnockOut™ |
| Product Size: | 500 mL |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Dry Ice |
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文献和实验Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
serum has been found to give desired mitotic division of lymphocytes of several animal species for chromosome evaluation. Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies B. R. Yadav
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
分化阶段的细胞(例如干细胞与定向前体细胞相比)需要不同的配方,对生长因子和细胞因子的选择尤为重要。细胞在无血清培养基中易受某些机械因素和化学因素的影响,培养基的保存和应用不如传统的合成培养基方便。而且在去除血清的同时,也去除了一些血清蛋白具有的保护、解毒作用,因此对试剂、水的纯度和仪器清洁度的要求更高。 目前市场上部分血清替代品: KnockOutTM Serum Replacement (Gibco) 胚胎干细胞(ESC
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