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- 百奥莱博
- GS4670-ZFE
- 北京
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
151
- 英文名：
Recombinant Human Interferon-alpha1a
- 保质期：
长期
- 供应商：
北京百奥莱博科技有限公司
- 保存条件：
冷冻(-20℃)
- 规格：
20μg
特别提示：包括重组人IFN-α1a在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
产品名称：重组人IFN-α1a
英文名称：Recombinant Human Interferon-alpha1a
产品货号：GS4670
产品规格：20μg
分子量：Approximately 19.5kDa,a single non-glycosylated polypeptide chain containing 167 amino acids.
储存条件：冷冻（-20℃）
概述
IFN-αs are proteins secreted by leukocyte.They are mainly involved in innate immune response against viral infection.The IFN-α family has 13 subtypes and 23 different variants.The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids.All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable.Many IFN-alpha subtypes differ in their sequences at only one or two positions.Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞97%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The specific activity determined by an anti-viral assay is no less than 1.0×108 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered solution in PBS,pH 7.4,containing 3%Mannitol,5%Trehalose,0.05%Tween-80. |
| 内毒素 | Less than 0.1 EU/μg of rHuIFN-α1a as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE |
我公司销售的重组人IFN-α1a价格，重组人Interferon-alpha1a，rInterferon-alpha1a，Recombinant Human Interferon-alpha1a，重组Interferon-alpha1a，rhInterferon-alpha1a，IFN-α1a, Human质量上佳，价格普惠，欢迎广大新老客户踊跃订购。
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文献和实验的培养及鉴定 2.1 将PBMC按1-2 x 106/ml的浓度悬浮于无血清培养液中，加入1,000 U/ml 的重组人IFN-g，37oC，5%CO2培养箱中培养； 2.2 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2，刺激CIK 细胞的生长和增殖； 注：此时也可同时加入100 U/ml的重组人IL-1a。 2.3 每3天半量换液或扩瓶一次，并补加重组人IL-2 300 U/ml； 2.4 在培养的第14d，收获CIK细胞。
Genotype 1a HCV (H77S) Infection System
therapy, based on the use of interferon-α (IFN-α) in combination with ribavirin, results in limited success, especially in patients infected with the most prevalent genotype 1 viruses. Better therapies are needed, but the inability to propagate HCV in cell
x 106/ml； 3.4 加入1 000 U/ml 的重组人IFN-g培养； 3.5 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2，刺激CIK 细胞的生长和增殖； 注：此时也可同时加入100 U/ml的重组人IL-1a。 3.6 每3天半量换液或扩瓶一次，并补加重组人IL-2 300 U/ml； 3.7 在培养的第7d，收获CIK细胞，此时数量应达到1x 109个以上。 3.8 CIK细胞质控： 3.8.1 台盼蓝染色检测
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