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文献和实验High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
) 0.05 mg/ml propidium iodide (PI) in phosphate‐buffered saline (PBS; appendix 2A ): 800 µl per plate Compounds for cytotoxicity testing (1 to 10 mM in DMSO) in a stock
Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
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