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文献和实验Transient transfection into 293T cells
Purpose Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
Reagent, TR-10 10-Liter Transient transfection • BrightLine Hemacytometer, Sigma, • Trypan Blue Solution (0.4%), Sigma, • 0.22 um GP Express Membrane Filter, Millipore IV. GENERAL CONSIDERATIONS A. Prior to Startup Since 293 cells tend
Transient transfection into 293T cel
Transient transfection into 293T cells(by Jun Takagi, Jun 15,2000)PurposeTransient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal
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