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文献和实验ChIP‐Seq: A Method for Global Identification of Regulatory Elements in the Genome
. The process is initiated by cross?linking DNA and DNA?bound proteins. Subsequently, chromatin is isolated from nuclei and subjected to sonication. An antibody against a specific TF or DNA?binding protein is then used to immunoprecipitate specific DNA?TF
Strategies to Optimize Protein Expression in E. coli
. 2009a. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114. Lefebvre, J., Boileau, G
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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