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- 详细信息
- 文献和实验
- 技术资料
- 库存:
438
- 英文名:
Recombinant Rat Vascular Endothelial Growth Factor165
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
2μg
特别提示:包括重组大鼠VEGF165在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组大鼠VEGF165
英文名称:Recombinant Rat Vascular Endothelial Growth Factor165
产品货号:GS4583
产品规格:2μg
分子量:Approximately 38.7kDa,a disulfide-linked homodimeric protein,consisting of two 165 amino acid polypeptide chains.
储存条件:冷冻(-20℃)
概述
Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells,which stimulates vasculogenesis and angiogenesis.VEGFs normal function is to create new blood vessels during embryonic development,new blood vessels after injury,muscle following exercise,and new vessels(collateral circulation)to bypass blocked vessels.Mouse and rat express alternately spliced isoforms of 120,164,and 188 amino acids(a.a.)in length.Recombinant Rat VEGF165 contains 165 amino acids residues and it is a disulfide-linked homodimer.In addition,it shares 97%a.a.sequence identity with corresponding regions of mouse,88%with human and bovine,89%with porcine and canine,and 90%with feline and equine VEGF,respectively.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC)is less than 5ng/ml,corresponding to a specific activity of>2.0×105 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 20mMTris,300 mM NaCl,pH 8.8. |
| 内毒素 | Less than 1 EU/μg of rRtVEGF165 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
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