DBP2 Pure 24/DBP2 150ug

E.Z.N.A. Cycle-Pure Kit Spin Protocol

of the sample at 260nm and then at 280nm. The DNA concentration is calculated as follows: 260 DNA concentration = A x 50 x (Dilution Factor) ug/ml Fragments greater than 500bp in length can routinely be purified at > 80% yield. 260 Bands ranging

Transgenic Mouse Core Facility

-ME and hydroxyquinoline. Vortex, RTo X 3min. Add an equal volume of 24:1 CHCl3/Isoamyl Alcohol. Vortex, spin in clinical centrifuge at speed 5 for 5 min. at RTo. (between 1400-1600 G) Remove aq. layer to a new tube, discard organic layer. Repeat steps D

免疫球蛋白编码基因的转录和翻译

子与启动子序列的距离缩短(在2kb范围内)，加强了增强子对启动子的顺式激活作用(cisacting)。在淋巴细胞特异性DNA结合蛋白(DBP)如OTF2A、OTF2B作用下，增强子可促进Ⅱ型RNA聚合酶与可变区基因上游5，端的启动子高保守序列TATAb。x结合，从而启动／z基因的转录，产生初级mRNA。转录的初级mRNA中仍然含有内含子等非编码序列，需经mRNA 5’端加帽、3，端多聚腺苷酸化、甲基化等碱基修饰、mRNA拼接(内含子切除)等转录后加工，成熟的mRNA从细胞核释放出来，随后在核糖体上进