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文献和实验三句话读懂一篇 CNS！首次提取超 100 万年的 DNA；轻轻一喷，瞬时改变作物性状...
://doi.org/10.1016/j.cell.2021.01.0222. Watson, M.J., Vignali, P.D.A., Mullett, S.J. et al. Metabolic support of tumour-infiltrating regulatory T cells by lactic acid. Nature (2021). https://doi.org/10.1038/s41586-020-03045-23. van der Valk, T., Pečnerová
T-cell dependent antigen specific in situ staining (Pe)
Double staining for Ag and Ki-67, TUNEL or BCL-6. Cut frozen tissue sections (spleen, lymph nodes) in a cryostat at ~ 4µm, on plain glass slides (Adhesive -coated slides are sometimes too hydrophobic, but fine
A general protocol for staining cell for cytometry analysis
(Cat. No. 66121A) : 0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1X prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1X PBS buffer. Store at RT. Recommended for use in parallel with Annexin
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