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文献和实验A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
a background and then counted as specific spots. We have tested a cell detachment reagent, Accumax™, and found that it may be used to remove a large number of cells adhered to the microplate membranes. Accumax was tested in 16 different ELISPOT assays
Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tube
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
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