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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
1). Add appropriate amount of lysis buffer forDNA extraction and incubate at 56°C overnight with shaking. 2. Add an equal amount of Tris-buffered phenol to samples. Mixthoroughly by inversion. Centrifuge at 2,500 × g for 5–10 min. 3. Transfer
., Ehsani, A., Salvaterra, p., and Rossi, J., Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells, Nat. Biotechnol., 20, 500, 2002. 14. Miyagishi, M. and Taira, K., U6 promoter-driven siRNAs with t~ur uridine
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