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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG1, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
PerCP/Cyanine 5.5
- 适应物种:
Human
- 保质期:
12个月
- 目录编号:
E-AB-F1195L
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Mouse
- 应用范围:
FCM
- 抗体英文名:
Elab Fluor® 488 Anti-Human CD28 Antibody[CD28.2]_货号:E-AB-F1195L
- 抗体名:
Elab Fluor® 488 Anti-Human CD28 Antibody流式抗体[CD28.2]_货号:E-AB-F1195L
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥2640.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥912.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥3960.0 |

Elab Fluor® 488 Anti-Human CD28 抗体流式抗体[CD28.2]产品名称:Elab Fluor® 488 Anti-Human CD28 Antibody[CD28.2], 货号:E-AB-F1195L
| 产品描述: | CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. |
| Alternate Names | PAS2;MNS;HGpMiX;GYPA;GPSAT; |
| Swissprot | P10747 |
| Gene ID | 940 |
| Clone No | CD28.2 |
| Host | Mouse |
| Reactivity | Human |
| Application | FCM |
| 同种型 | Mouse IgG1, κ |
| 同种型 Control | Elab Fluor® 488 Mouse IgG1, κ 同种型 Control[MOPC-21] |
| Conjugation | Elab Fluor® 488 |
| Conjugation Information | Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter). |
| Spectrum | |
| 储存方法 缓冲 | Phosphate 缓冲ed solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
| 储存方法 | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
| Expiration date | 12个月 |
| Shipping | 冰袋 |

Elab Fluor® 488 Anti-Human CD28 抗体流式抗体[CD28.2]labscience®拥有先进的单抗,多抗和流式抗体开发技术平台,提供8500+高品质 抗体, 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证,包含 流式细胞术(Flow Cytometry)、 免疫印迹(WB)、 免疫组化(IHC/ICC)、 免疫(共)沉淀(IP/Co-IP)、 免疫荧光(IF) 等实验。抗体产品涉及多种生命科学研究领域,包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类,以保障并加快您的研究进程
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.Elab Fluor® 488 Anti-Human CD28 抗体流式抗体[CD28.2]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
Vybrant® DyeCycle Green and Orange Stains
degradation. Incubate at 37?C for 30 minutes, protected from light. For cells stained with Vybrant® DyeCycle™ Green stain, analyze samples on flow cytometer using 488 nm excitation and green emission (Figure 3). For cells stained with Vybrant®
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