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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PE/Cyanine5 Mouse IgG2b, κ Isotype Control同型对照[MPC-11]_货号:E-AB-F09812G
- 抗体英文名:
PE/Cyanine5 Mouse IgG2b, κ Isotype Control[MPC-11]
- 应用范围:
FCM
- 宿主:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F09812G
- 级别:
科研级别
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 克隆性:
MPC-11
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态:
液体
- 亚型:
Mouse IgG2b, κ
- 规格:
100Tests/20Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥1800.0 |
|---|---|---|---|
| 规格: | 20Tests | 产品价格: | ¥600.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥2880.0 |
货号:E-AB-F09812G
| Clone No |
MPC-11
|
| Host |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG2b, κ
|
| Conjugation |
PE/Cyanine 5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
应该是APC标记的Mouse IgG1,κ,货号是555751。注意同型对照的成份跟它的名称是相同的.如果是抗体的组合形式是纯化的一抗+荧光标记的二抗,那么应该选择一抗的同型对照。比如CDw125的纯化抗体,货号是555901,成分是Mouse IgG1,κ。它的同型对照是纯化的Mouse IgG1,κ,货号是555746。它的二抗PE标记的抗小鼠IgG1,货号是550083。那么染色方式如下: 样本管:纯化的CDw25+PE标记的抗Mouse IgG1+
Immunofluorescent Staining of Mouse and Rat Leukocytes
cold wash buffer (PBS/0.1% NaN3/1.0% fetal bovine serum). Centrifuge at 350 x g for 5 min. Finally, resuspend cell pellet to a concentration of 2 x 107 cells/ml (i.e., 106 cells per 50 µl). Dilute primary mAbs (e.g., unconjugated, biotinylated
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