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MCE 国际站:SYBR Green qPCR 预混液 (无 ROX)
SYBR Green qPCR Master Mix (No ROX) 是一种 2× 浓度的即用型预混液,含有 qPCR 的所有组分, 只需加入样品 DNA,引物和水即可快速高效完成 qPCR 反应。
Description & Advantages:
The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer and ddH2O.
MCE qPCR Master Mix (No ROX) can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. No ROX Reference Dye is included for use with instruments that does not require a reference dye for rectify the error of fluorescence signals between different wells.
MCE qPCR Master Mix (No ROX) is applied for the Real-Time PCR instrument as follows:
Bio-Rad: CFX96, CFX384, iCycler IQ, MyiQ, iQ5, MiniOpticon, Opticon, Opticon 2 and 4;
Eppendorf: Mastercycler ep realplex, realplex2 s;
Qiagen: Corbett Rotor-Gene Q, Corbett Rotor-Gene 3000, Corbett Rotor-Gene 6000;
Roche: LightCycler 480, LightCycler 2.0; LightCycler 96;
Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.
1. Ready to use.
2. Optimized buffer enhances specificity and reduces primer-dimer formation.
3. High sensitivity, specificity and reliability.
4. No ROX Reference Dye is included.