细胞衰老 β-半乳糖苷酶染色试剂盒

细胞衰老 β-半乳糖苷酶染色试剂盒 MCE 细胞衰老 β-半乳糖苷酶染色试剂盒可用于观察细胞或组织的衰老情况。 产品来源： www.medchemexpress.cn/inhibitor-kit/cell-senescence-β-galactosidase-staining-kit.html Description & Advantages: Due to limited replicative lifespan, normal cells enter cell cycle arrest, also known as cellular senescence1. While in this senescence phase the cells remain metabolically active without undergoing cell death or division. These senescent cells adopt a specific phenotypic state that includes the appearance of multinucleated cells, increased vacuolization, expression of pH-dependent β-galactosidase, and morphological changes where cells become enlarged and extended2, 3. Senescence, through a variety of mechanisms, can also play a role in tumor suppression, tumor progression, aging, and tissue repair. β-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene (lacZ) in the presence of a β-gal substrate to identify recombinant clones. In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. MCE Cell Senescence β-Galactosidase Staining Kit is based on SA-β-gal catalyzing the transformation of X Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, X-gal) into a blue product at pH 6. This allows for the visualization of cell or tissue senescence under the microscope. 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种TOP期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪前沿的制药及生命科学研究进展，为您提供全球前沿的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。