Nervonic acid神经酸,506-37-6

Vybrant® DyeCycle Green and Orange Stains

material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

8) for 2 h at 37°C. Load 20 µL (~20 µg) of the trypsin-digested phosphorylated protein with 30 µL of 100 mM acetic acid onto the IMAC column. Incubate the column on a slowly rotating wheel for 30 min at 37°C. Check the column periodically for leakage

Two dimensional peptide mapping

will probably go down. After exposure, cut out the membrane piece of interest and soak it immediately in 0.5% PVP-360 (Polyvinylpyrrolidone, MW360) in 100mM acetic acid for 30 min. at 37°C. If you don't do this, your yield will be poor. This may result