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- GE
- 28995043
- 2025年07月16日
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文献和实验Studies of the Ubiquitin Proteasome System
) Kit for first‐stand cDNA synthesis (e.g., RT‐PCR kit; Fermentas) Kit for PCR amplification of DNA (e.g., RT‐PCR kit, Fermentas, or Ready‐To‐Go PCR beads, GE Healthcare) Primers for PCR
. 6. 10× Tango buffer (Thermo Scientifi c/Fermentas). 7. DTT (DL-Dithiothreitol; Cleland’s reagent). 8. 10 mM Adenosine 5′-triphosphate. 9. Ultrapure water (RNAse/DNAse-free). 10. Tris-EDTA (TE) buffer. 11. E. coli competent cells. Strains
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Transfection Kit (Clontech) CD34+ cell separation kit (CD34 MicroBead Kit containing anti-human CD34 MicroBeads and Blocking Reagent; Miltenyi Biotec) Cellgro medium (serum-free medium; Cellgenix) DMEM for HSC Fetal calf serum (FCS
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