- ￥2880
- 瑞典Agrisera
- 瑞典
- AS10 664
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Goat anti-Human IgG Fc, HRP conjugated, min. reactivity to human IgA and IgM
- 规格：
1mg
| Immunogen |
| affinity purified human IgG Fc part |
|
Host Goat
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Clonality Polyclonal
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Purity Affinity purified IgG
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Format Lyophilized
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|
Quantity 1 mg
|
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage Store lyophilized material at 2-8°C.
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| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
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Recommended dilution 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 100 000 (WB)
|
名称：Goat anti-Human IgG Fc, HRP conjugated, min. reactivity to human IgA and IgM
货号：AS10 664
品牌：Agrisera
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
可能与其他物种发生交叉反应， 除非特异性的经过交叉反应物种的吸附处理。在抗体描述中，经过其他物种的吸附处理的抗体标有“ (min X …Sr Prot) ”字样 。 Anti-IgG (H+L) 这类抗体和 IgG 分子的重链和轻链都可以反应，比如，和 IgG 分子的 Fc ， F(ab ’)2/Fab 部分。 Anti-IgG (H+L) 抗体也可以和其他的免疫球蛋白家族（ IgM ， IgA ，IgD,IgE ）和亚家族反应，因为所有的免疫球蛋白都有相同的轻链（或者 κ 链
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