Donkey anti-Goat IgG (H&L), HR
P conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG抗体- ￥3244
- 瑞典Agrisera
- 瑞典
- AS10 1069
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Donkey anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG
- 规格：
1mg
| Host Donkey |
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Clonality Polyclonal
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Purity Affinity purified IgG
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Format Liquid
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Quantity 1 mg (1.0 mg/ml)
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Storage Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Recommended dilution The optimal working dilution should be determined by the investigator.
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名称：Donkey anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG
货号：AS10 1069
品牌：Agrisera
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文献和实验blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
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