- ￥17479
- Agrisera
- 瑞典
- AS16 ECL-SN-1L
- 2025年07月06日
企业认证
相关产品推荐更多 >
Anti-Mouse CD45, APC (Clone:30-F11)检测试剂
￥268.80
TSH (beta)抗体
￥7412Goat anti-Rat IgG (H&L), ALP conjugated抗体
￥2880Anti-Human CD23, PE(Clone:EBVCS2)检测试剂
￥672
Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, DyLight® 633 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG
￥3441
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Agrisera ECL kit (Bright/SuperBright) (1L)
- 规格：
1kit
| Quantity Agrisera ECL SuperBright: 2 x 500 ml, two component ready to use solutions, enough for 500 midi blots (6.8 x 8.1 cm). |
| Agrisera ECL Bright: 2 x 500 ml, two component ready to use solutions, enough for 500 midi blots (6.8 x 8.1 cm). |
|
Storage
|
| 18 month shelf life (Agrisera ECLSuperBright) at 2°C to 8°C. |
| 24 month shelf life (Agrisera ECLBright) at 2°C to 8°C. |
| Mixed working reagent is stable for several days at room temperature or at 4°C. |
| Exceptional lot to lot consistency. |
|
Tested applications Western blot (WB)
|
名称：Agrisera ECL kit (Bright/SuperBright) (1L)
货号：AS16 ECL-SN-1L
品牌：Agrisera
点击查看产品详细说明书
更多产品详情：请向 <上海经科化学科技有限公司-客服> 索取
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Secondary Antibody to the dilution of 1:2000 (probably AP-labelled, sheep anti-mouse IgG), incubate at room temperature for ~30 minutes. 5. Wash filters with two quick rinses, then 3X 5 minutes with TBSt. 6. To develop with ECL Chemifluorescent Kit: -mix
from the kit to the column. 17. Let stand for 1 minute. 18. Centrifuge at 2880 x g for 5 minutes. 19. Add another 1 ml of water to the column. 20. Let stand for 1 minute. 21. Centrifuge at 2880 x g for 10 minutes. 22. Aliquot
by autoclaving.AAA. 封闭，一抗，二抗时的温度有没有什么规定呢，比如现在我就在室温里做，或者要在4度下?解答：均可在室温进行，如果时间不够，一抗孵育可以先在室温进行一个小时，然后4度过夜。BBB. 实验室暂无NP40我用sds可以吗？另外有过用尿素和硫脲提取膜蛋白吗？配方如下：7M 尿素，2M 硫脲，Triton-x-100 0.2ml，新鲜加入：65mM DTT蛋白酶抑制剂：试剂盒HaltTM Protease inhibitor cocktail kit 1%(v/v)是用于抽提双向电泳用蛋白的配方
技术资料暂无技术资料 索取技术资料
