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- 详细信息
- 文献和实验
- 技术资料
- 库存:
进口期货
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
RpoB | RNA polymerase beta subunit (chloroplast) (maize)
- 规格:
100ug
| Immunogen His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3 |
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Host Rabbit
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Clonality Polyclonal
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Purity Affinity purified serum in PBS, pH 7.4
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Format Lyophilized in PBS pH 7.4
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Quantity 100 µg
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Reconstitution For reconstitution add 50 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution 1 : 500 (WB)
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Expected | apparent MW
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| 121 kDa |
名称:RpoB | RNA polymerase beta subunit (chloroplast) (maize)
货号:AS15 2867A
品牌:Agrisera
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文献和实验Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification
For patients infected with tuberculosis, detection of rpoB gene mutations is critical for diagnosing drug-resistant strains of the causative pathogen, Mycobacterium tuberculosis (MTB). Traditional approaches to drug resistance include culture
种耐药性均涉及结核分枝杆菌一个或多个基因的突变、缺失或颠换等。 抗结核药物RFP的主要作用是抑制结核分枝杆菌依赖DNA的RNA聚合酶,抑制转录过程,导致细胞死亡。96%RFP耐药菌株编码RNA聚合酶β亚单位的rpoB基因78bp区域(508―533位)发生突变,碱基插入或缺失使RFP与RNA聚合酶亲和力明显减小,是结核分枝杆菌耐RFP的主要机制。研究表明,rpoB基因突变分布在78个碱基26个氨基酸范围内,突变频率最高的是氨基酸密码子531,次之为526及516。尽管有
Sequence-Based Identification of Legionella
analysis tool. Other gene targets are available for most if not all species, the best of which target 16S rRNA, rpoB, rnpB , or proA genes. One or several of these should be used to confirm important strains or clarify apparent novelness. The increased
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