- ￥1990 - 2990
- KA&M BIO
- BW4765
- 2025年07月10日
- WB, IP
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
详见说明
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
at-20℃
- 库存：
99
- 供应商：
上海圻明生物
- 应用范围：
WB, IP
- 浓度：
500ug/ml
- 抗体英文名：
RNA polymerase beta/RPOB Rabbit mAb
- 抗体名：
RNA polymerase beta/RPOB单克隆抗体
- 规格：
100μl
RNA polymerase beta/RPOB Rabbit mAb抗体上海圻明生物优势供应。更多具体产品详细说明资料欢迎免费咨询客服。
Anti-GAPDH Mouse mAb(GA1R)说明书
DESCRIPTION: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
SPECIFICITY: H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh[1]
ISOTYPE: IgG1
MOLECULAR WEIGHT: 37KDa
TESTED APPLICATIONS: Western Blot, Immunoprecipitation, Immunofluorescense IP ELISA
APPLICATIONS: Western blot: 1:1,000-1:8,000
Immunoprecipitation or immunostaining: 1:500-1：2,000
ELISA on recombinant proteins: 1:500-1:2,000
*NOTE: Optimal working dilutions must be determined by the end user
STORAGE/HANDING: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
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欢迎咨询选购优势产品RNA polymerase beta/RPOB Rabbit mAb抗体
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文献和实验相关专题 重组DNA技术工具酶 RNA聚合酶(RNA polymerase)：以一条DNA链或RNA为模板催化由核苷-5′-三磷酸合成RNA的酶。 催化转录的RNA聚合酶是一种由多个蛋白亚基组成的复合酶。如大肠杆菌的 RNA聚合酶有五个亚基组成，其分子 量为480000，含有α，β，β’，δ等4种不同的多肽，其中α为两个分子，所以全酶(holoenzyme
Nucleotide Analogues as Probes for DNA and RNA Polymerases
typically used, the experimental approaches with different polymerases, and issues with data interpretation. Curr. Protoc. Chem Biol. 2:111?124. © 2010 by John Wiley & Sons, Inc. Keywords: polymerase; nucleotide; DNA; RNA; kinetics
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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