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60
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深圳子科生物科技有限公司
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25 Reactions
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文献和实验Reverse Ligation Mediated RT -
the 2-4µl of supernatant so collected. Resuspend the precipitates in 4µl H 2 O. Store on ice until ready to proceed. Make RT mastermix 1 with the following components and with this volume per reaction 0.9µl H 2 O
Reverse Ligation Mediated RT - PCR
. Brief second spin, pulse to full speed then release, discard the 2-4µl of supernatant so collected. Resuspend the precipitates in 4µl H2 O. Store on ice until ready to proceed. Make RT mastermix 1 with the following components
) 1 m l template RNA (50 pg-1 m g) 1 m l nucelase-free ddH2 O 15.7 m l 2. Setup master mix 2: 5X RT-PCR buffer
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