- ￥1546
- Agrisera
- 瑞典
- AS08 300
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
1 | PEB (4x) | protein extraction buffer
- 规格：
5x2ml
| Quantity 5 x 2 ml (4x stock) allows up to 75 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll) |
|
Storage Stable at RT for at least 1 month; short-term storage (6 month) at 4°C and long term storage (1 year or more) at -20°C
|
|
Tested applications protein extraction
|
名称：1 | PEB (4x) | protein extraction buffer
货号：AS08 300
品牌：Agrisera
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文献和实验Protocol for Protein Extraction
Protocol for Protein Extraction 10 % w/v TCA/ acetone/ 0.07 % v/v -Mercaptoethanol Plant cells are rich in compounds that interfere with the 2DE separation method such as salts, organic acids, phenolics, pigments, terpenes
Protein extraction from whole tissues for IEF
after removing as much of the ethanol as possible with a Pipetman. Resuspend final pellet in an appropriate (minimum) volume of IEF Sample Buffer and store at -20 C. protein extraction solutions 1 M Tris-HCL (pH 8.8) - 78.8g/500 mls Tissue
Protocol of the protein extraction method
of the protein. Annotation: the component of buffer: Lysis bufferⅠ (250ml): Glycerd: 25ml (10%) Triton X-100: 2.5ml (1%) Hepes (PH7.5): 12.5ml (50mM) NaCl (150mM): 37.5ml NaF (2mM) EDTA
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