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人长R3胰岛素样生长因子I(LR3IGF-I) ELISA试

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  • ¥5000
  • biosensis
  • BEK-2233-1P/2P
  • 2025年08月28日
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      96T/kit

    人长R3胰岛素样生长因子I(LR3IGF-I) ELISA试剂盒
    IGF-1又称促生长因子C,属于胰岛素基因家族(包括胰岛素和松弛素),在结构上与胰岛素前体蛋白相类似并具有更高的促生长的功能。血液中的IGF-1由肝脏合成,并受外周的GH调控分泌于血液中,介导GH的促生长功能。另外IGF-1能促进多数细胞的分裂、迁移和分化,调节糖和脂肪的代谢,以及调节固醇类激素的活性、软骨和骨代谢,并在肌肉的重建和肿瘤的生长过程中有重要的作用。IGF-1能与IGF-RIGF-R和胰岛素受体结合,血液中与IGF-1结合蛋白结合从而延长自身的半衰期。

    人长R3胰岛素样生长因子I (Long-R3-IGF-I, LR3IGF-I) 是一个含有83个氨基酸的人IGF-I的类似物。与完整的人IGF-I相比,在第3个位点氨基酸由Arg对替换天然的Glu(因此称为R3,并在N端增加了13个氨基酸的延伸肽。人Long-R3-IGF-I显著提高了人天然的IGF-I生物学活性,因为人Long-R3-IGF-I大大降低了与人IGF-I结合蛋白的结合能力,而这种结合通常是限制IGFs的生物学活性的。


     
    LR³IGF-I Rapid™ ELISA试剂盒的开发汇聚了 GroPep公司在IGF研究领域多年的专业经验以及Biosensis公司建立的 Rapid™ ELISA平台开发经验。试剂盒可在不到3小时内特异性的定量检测 LR³IGF-I 蛋白水平,灵敏度高,结果可靠。

    LR³IGF-I Rapid™ ELISA试剂盒提供整套试剂以及预包被的酶标板,可实现快速检测培养基中LR³IGF-I 的含量,试剂盒组分包括LR³IGF-I 单克隆捕获抗体(预包被于酶标板) LR³IGF-I蛋白标准品、LR³IGF-I 生物素化抗体、辣根过氧化物酶-链亲和素结合物、TMB底物以及洗涤缓冲液等。
    Description Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents  specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis.

    This LR3IGF-I RapidTM ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established RapidTM ELISA platform. This collaboration has resulted in the new LR3IGF-I RapidTM ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours!

    The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti-LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti-LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard.

    This LR3IGF-I RapidTM ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
    Batch No. Refer to the product label.
    Antigen LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
    Other Names Human LR3 insulin-like Growth Factor-I.
    Accession Q9NO10 IGF1_HUMAN (unmodified protein)
    Specificity Human
    Cross-reactivity The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
    Storage Store at 2-8C
    Expiry Date 12 months from purchase.
    Kit components The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
    Range 3.9 ng/mL - 200 ng/mL
    Sensitivity Typical limit of detection (LOD) for LR3IGF-I is 1 ng/mL determined as 150% of the blank value.

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        上海西唐生物科技有限公司   021-55229872,  65333639   www.westang.com 兔胰岛素样生长因子-1 ( IGF-1 )ELISA 试剂盒   ( 用于血清、血浆、细胞培养上清液和其它生物体液内 )   原理 本实验采用双抗体夹心  ABC-ELISA 法。用抗兔   IGF-1   单抗包被于酶标板上,标准品和样品中的   IGF-1与单抗结合,加入生物

    • 胰岛素样生长因子-1(IGF-1)ELISA试剂盒 说明书

      上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 狗胰岛素样生长因子 -1(IGF-1)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗狗 IGF-1 单抗包被于酶标板上,标准品和样品中的 IGF-1 与单抗结合,加入生物素化的抗狗 IGF-1 ,形成免疫复合物连接在板上

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