相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 适应物种:
人
- 规格:
96T/kit
IGF-1又称促生长因子C,属于胰岛素基因家族(包括胰岛素和松弛素),在结构上与胰岛素前体蛋白相类似并具有更高的促生长的功能。血液中的IGF-1由肝脏合成,并受外周的GH调控分泌于血液中,介导GH的促生长功能。另外IGF-1能促进多数细胞的分裂、迁移和分化,调节糖和脂肪的代谢,以及调节固醇类激素的活性、软骨和骨代谢,并在肌肉的重建和肿瘤的生长过程中有重要的作用。IGF-1能与IGF-ⅠR、IGF-ⅡR和胰岛素受体结合,血液中与IGF-1结合蛋白结合从而延长自身的半衰期。
人长R3胰岛素样生长因子I (Long-R3-IGF-I, LR3IGF-I) 是一个含有83个氨基酸的人IGF-I的类似物。与完整的人IGF-I相比,在第3个位点氨基酸由Arg对替换天然的Glu(因此称为R3),并在N端增加了13个氨基酸的延伸肽。人Long-R3-IGF-I显著提高了人天然的IGF-I生物学活性,因为人Long-R3-IGF-I大大降低了与人IGF-I结合蛋白的结合能力,而这种结合通常是限制IGFs的生物学活性的。
LR³IGF-I Rapid™ ELISA试剂盒的开发汇聚了 GroPep公司在IGF研究领域多年的专业经验以及Biosensis公司建立的 Rapid™ ELISA平台开发经验。试剂盒可在不到3小时内特异性的定量检测 LR³IGF-I 蛋白水平,灵敏度高,结果可靠。
LR³IGF-I Rapid™ ELISA试剂盒提供整套试剂以及预包被的酶标板,可实现快速检测培养基中LR³IGF-I 的含量,试剂盒组分包括LR³IGF-I 单克隆捕获抗体(预包被于酶标板)、 LR³IGF-I蛋白标准品、LR³IGF-I 生物素化抗体、辣根过氧化物酶-链亲和素结合物、TMB底物以及洗涤缓冲液等。
| Description | Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I RapidTM ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established RapidTM ELISA platform. This collaboration has resulted in the new LR3IGF-I RapidTM ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti-LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti-LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I RapidTM ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | Refer to the product label. |
| Antigen | LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus. |
| Other Names | Human LR3 insulin-like Growth Factor-I. |
| Accession | Q9NO10 IGF1_HUMAN (unmodified protein) |
| Specificity | Human |
| Cross-reactivity | The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%. |
| Storage | Store at 2-8C |
| Expiry Date | 12 months from purchase. |
| Kit components | The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols. |
| Range | 3.9 ng/mL - 200 ng/mL |
| Sensitivity | Typical limit of detection (LOD) for LR3IGF-I is 1 ng/mL determined as 150% of the blank value. |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验兔胰岛素样生长因子-1(IGF-1)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔胰岛素样生长因子-1 ( IGF-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗兔 IGF-1 单抗包被于酶标板上,标准品和样品中的 IGF-1与单抗结合,加入生物
狗胰岛素样生长因子-1(IGF-1)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 狗胰岛素样生长因子 -1(IGF-1)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗狗 IGF-1 单抗包被于酶标板上,标准品和样品中的 IGF-1 与单抗结合,加入生物素化的抗狗 IGF-1 ,形成免疫复合物连接在板上
人胰岛素样生长因子-2(IGF-2)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人胰岛素样生长因子-2 ( IGF-2 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IGF-2 单抗包被于酶标板上,标准品和样品中的 IGF-2与单抗结合,加入生物
技术资料暂无技术资料 索取技术资料









