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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human TMEM106B protein
- 亚型:
IgG1
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, IP, ELISA
- 靶点:
TMEM106B (1-46aa)
- 抗体英文名:
TMEM106B (1-46aa) Monoclonal antibody
- 抗体名:
TMEM106B (1-46aa) Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | U2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60333-1-Ig targets TMEM106B (1-46aa) in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21448 Product name: Recombinant human TMEM106B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-46 aa of BC033901 Sequence: MGKSLSHLPLHSSKEDAYDGVTSENMRNGLVNSEVHNEDGRNGDVS |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 106B |
| 别名 | TMEM106B, 5D1F8, Transmembrane protein 106B |
| 计算分子量 | 31 kDa |
| 观测分子量 | ~40 kDa |
| GenBank蛋白编号 | BC033901 |
| 基因名称 | TMEM106B |
| Gene ID (NCBI) | 54664 |
| RRID | AB_2881442 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NUM4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | Cell Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.Authors - Andrew Chang | |
| human | WB | Brain C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypesAuthors - Cristina T Vicente |
| human | WB | Pathol Res Pract TESC acts as a prognostic factor and promotes epithelial-mesenchymal transition progression in esophageal squamous carcinomaAuthors - Yanxin Dong |
| human | WB,IP,IF | F1000Res The identification of high-performing antibodies for transmembrane protein 106B (TMEM106B) for use in Western blot, immunoprecipitation, and immunofluorescenceAuthors - Riham Ayoubi
|
| human | WB | FASEB J Lysosomal membrane protein TMEM106B modulates hematopoietic stem and progenitor cell proliferation and differentiation by regulating LAMP2A stabilityAuthors - Di Guo |
| Human | IF | Life Med Tracing TMEM106B fibril deposition in aging and Parkinson's disease with dementia brainsAuthors - Wanbing Zhao |
CD46 分子 CD46 常用单克隆抗体或代号: HULYM5,J48 主要表达细胞: 广泛,Leu,Pt [NL] 分子质量(kDa)和结构: gp56- 66,膜辅助因子蛋白(MCP) 功 能: 调节补体活化,裂解C3b ,C4b膜辅助因子蛋白 CD46 Aka membrane cofactor protein (MCP) Cofactor for Factor
如今,单克隆抗体市场炙手可热。一方面,由于巨大的应用价值,单克隆抗体需求强劲。另一方面,单克隆抗体制备工艺复杂、周期长。为此,能否快速制备出高质量的单克隆抗体已成为影响抗体研究应用的关键。46天制备出高质量的单克隆抗体在几年前在京天成生物技术(北京)有限公司得以实现,从而使之在全球制备高质量单克隆抗体服务竞争中占极大优势。 单克隆抗体具有理化性状高度均一、生物活性单一、与抗原结合的特异性强、便于人为处理和质量控制等特点。正是这些优点使它一问世就受到高度重视,并广泛应用于生物
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
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