- ￥1350 - 3000
- Proteintech
- 55201-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
SET/TAF-I
- 抗体英文名：
SET/TAF-I Polyclonal antibody
- 抗体名：
SET/TAF-I Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | mouse spleen tissue, human kidney tissue, human liver cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55201-1-AP targets SET/TAF-I in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SET nuclear oncogene |
| 别名 | 2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, SET/TAF-I, TAF I, TAF IBETA, Template activating factor I |
| 计算分子量 | 33 kDa |
| 观测分子量 | 41 kDa, 39 kDa |
| GenBank蛋白编号 | NM_003011 |
| 基因名称 | SET |
| Gene ID (NCBI) | 6418 |
| RRID | AB_10837360 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01105 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell growth and proliferation via many pathways that promote tumorigenesis and metastasis. The SET protein shows apparent molecular masses of 41 and 39 kDa (PMID: 12759364, PMID: 7753797).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Rep A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repressionAuthors - Ling Zhang
|
| human | WB | Oncotarget Overexpression of PP2A inhibitor SET oncoprotein is associated with tumor progression and poor prognosis in human non-small cell lung cancer.Authors - Hao Liu
|
| human | WB | J Med Chem Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.Authors - Brianna D Mackie |
| human | WB | Cancers (Basel) Pre-Clinical Study Evaluating Novel Protein Phosphatase 2A Activators as Therapeutics for Neuroblastoma.Authors - Laura V Bownes |
| human | WB | Cell Signal PMID: 25152373Authors - Hao Liu |
| human | WB,IHC | BMC Cancer MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer.Authors - Hongli Pan |
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
coupled with mass spectrometry (AP?MS) is a robust technique used to identify protein?protein interactions. With recent improvements in sample preparation, and dramatic advances in MS instrumentation speed and sensitivity, this technique
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
600, then dilute the cells to final OD600 of 0.5, place on ice. Screening Plaques with AP-Fusion Protein Reagents: 1M IPTG stock solution NZY plates with phage 20 x
检测方法 对于探针和靶序列杂交之后的检测，我们通常使用碱性磷酸酶（AP）耦联的抗体，采用显色法或化学发光法检测。在碱性磷酸酶的底物中，我们使用CDP-Star或CSPD用于化学发光法检测，NBT/BCIP用于显色法检测。同时，对于膜杂交中的化学发光信号的检测，建议使用Lumi-Film以获得最理想的信号。 检测底物 即用型CDP
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