- ￥1350 - 3000
- Proteintech
- 27883-1-AP
- 2025年10月13日
- WB, IF/ICC, FC (Intra), ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MDM2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, FC (Intra), ELISA
- 靶点：
MDM2/HDM2
- 抗体英文名：
MDM2/HDM2 Polyclonal antibody
- 抗体名：
MDM2/HDM2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | MCF-7 cells, A431 cells, A549 cells, HepG2 cells, T-47D cells |
| Positive IF/ICC detected in | MG132 treated U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27883-1-AP targets MDM2/HDM2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27255 Product name: Recombinant human MDM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-298 aa of NM_001145337.2 Sequence: MVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEIS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Mdm2 p53 binding protein homolog (mouse) |
| 别名 | MDM2, E3 ubiquitin-protein ligase Mdm2, EC:2.3.2.27, hdm2, p53-binding protein Mdm2 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 75-85 kDa, 57-60 kDa, 40-55 kDa |
| GenBank蛋白编号 | NM_001145337.2 |
| 基因名称 | MDM2 |
| Gene ID (NCBI) | 4193 |
| RRID | AB_2881003 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00987 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the cellular localization of MDM2?
MDM2 is predominantly localized in the nucleoplasm.
What is the tissue specificity of MDM2?
MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain
isoforms only express in cancerous cells and not normal tissue.
With what other molecules does MDM2 interact?
Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional
activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to
MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.
What competes with MDM2 activity?
Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.
What is the role of ubiquitination in MDM2 activity?
While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination
mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of
p53.
How is MDM2 associated with cancer?
MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in
promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development
and growth.
What is the human ortholog of MDM2?
Human double-minute type 2 (HDM2)
In what cellular circumstances does the p53-MDM2 complex dissociate?
Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also
prevents p53-MDM2 interaction.
Where is the gene located?
Hdm2 is located on human 12q15.
Mdm2 is located on murine 10 D2.
PMIDs: 24309898, 29584707, 9840926, 28714398
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | J Clin Invest ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.Authors - Hidemichi Kouzu |
| mouse,human | WB,IF | Cell Chem Biol Repurposing AS1411 for constructing ANM-PROTACsAuthors - Xuekun Fu |
| mouse | WB | Mol Metab Multi-Omics after O-GlcNAc Alteration Identified Cellular Processes Promoting Aneuploidy after Loss of O-GlcNAc TransferaseAuthors - Samuel S Boyd |
| human | WB,IF,CoIP | Eur J Med Chem Development of hybrid aptamers-engineered PROTACs for degrading VEGF165 in both tumor- and vascular endothelial cellsAuthors - Ziting Feng |
PLoS Pathog Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entryAuthors - Bing-Qian Su | ||
| human | WB | Bioorg Chem Design, synthesis and biological evaluation of novel pyrrolidone-based derivatives as potent p53-MDM2 inhibitors.Authors - Dongjuan Si |
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