- ￥1350 - 3000
- Proteintech
- 24412-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Barkor protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
ATG14/Barkor (C-terminal)
- 抗体英文名：
ATG14/Barkor (C-terminal) Polyclonal antibody
- 抗体名：
ATG14/Barkor (C-terminal) Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells |
| Positive IHC detected in | human brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24412-1-AP targets ATG14/Barkor (C-terminal) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag19707 Product name: Recombinant human Barkor protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 152-406 aa of BC109090 Sequence: YSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESME |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0831 |
| 别名 | Barkor, ATG14, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Atg14L |
| 计算分子量 | 492 aa, 55 kDa |
| 观测分子量 | 65-68 kDa, 42 kDa |
| GenBank蛋白编号 | BC109090 |
| 基因名称 | ATG14 |
| Gene ID (NCBI) | 22863 |
| RRID | AB_2879531 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZNE5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification. A major band around 65-68 kDa and a light band around 42 kDa can be observed in western blot which may be caused by alternative splicing of ATG14. The specificity of ATG14 antibody has been tested by siRNA test.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Cell Death Differ ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.Authors - Isaac Tamargo-Gómez |
| human | IF | J Cell Sci Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms.Authors - Shun Kato |
| human | WB | Microb Pathog Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells.Authors - Qiang Fu |
| mouse | WB,IF | Exp Eye Res Inhibiting miR-129-5p alleviates inflammation and modulates autophagy by targeting ATG14 in fungal keratitis.Authors - Jingqiong Lin |
| human | WB | EMBO Rep An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migrationAuthors - Peigang Liang |
Regulation of AP-1 Activity by TGF
with each other or Fos family members, which cannot bind DNA on their own. The dimerization, which is mediated by a leucine zipper motif, allows the complex to bind to a consensus DNA sequence 5′-TGA G/C TCA-3′, termed an AP-1 site. This same sequence
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人转录因子AP-1 ( AP-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 AP-1 单抗包被于酶标板上，标准品和样品中的 AP-1与单抗结合，加入生物素化的抗人 AP
and 10X PCR buffer. PCR program: 95¡C X 3'; 30 X (95¡C X 1', 55¡C X 1', 72¡C X 3');72¡C X 3' PCR for FGF9 KO mice Oligos... 5’ oligo: (JC46): 5’-GCA AGG GAG GGG AGT TGG ATA TAC C-3’ 3’ oligo: (JC42): 5’-CAG CCC AAG CTT TCG CGA GCT CG
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