- ￥1350 - 3000
- Proteintech
- 23705-1-AP
- 2025年10月13日
- IHC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-19 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, ELISA
- 靶点：
IL-19
- 抗体英文名：
IL-19 Polyclonal antibody
- 抗体名：
IL-19 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23705-1-AP targets IL-19 in IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag18887 Product name: Recombinant human IL-19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 63-215 aa of BC153127 Sequence: LRRCLISTDMHHIEESFQEIKRAIQAKDTFPNVTILSTLETLQIIKPLDVCCVTKNLLAFYVDRVFKDHQEPNPKILRKISSIANSFLYMQKTLRQCQEQRQCHCRQEATNATRVIHDNYDQLEVHAAAIKSLGELDVFLAWINKNHEVMFSA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 19 |
| 别名 | IL 10C, IL 19, IL19, IL-19, interleukin 19, MDA1, NG.1, ZMDA1 |
| 计算分子量 | 177 aa, 20 kDa |
| GenBank蛋白编号 | BC153127 |
| 基因名称 | IL-19 |
| Gene ID (NCBI) | 29949 |
| RRID | AB_2879308 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHD0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-19, a member of the IL-10 cytokine family (IL-10, -19, -20, -22, -24, -26, -28, and -29), is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-α production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Inflamm Res Interleukin-19 upregulates fibronectin and collagen I expression via the NF-κB-Smad2/3 pathway in fibroblasts of patients with chronic rhinosinusitisAuthors - Hongwei Bao |
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人转录因子AP-1 ( AP-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 AP-1 单抗包被于酶标板上，标准品和样品中的 AP-1与单抗结合，加入生物素化的抗人 AP
1. 免疫动物：两只新西兰兔2. 佐剂：首先用完全弗氏佐剂（CFA），后用不完全弗氏佐剂（IFA）。3. 免疫原：蛋白或KLH偶联的多肽。每次免疫100-200μg免疫原。4. 免疫：用生理盐水稀释免疫原，然后与相应的佐剂进行1:1混合。抗原和佐剂完全混合形成稳定的乳剂，将该乳剂在兔子双肩周围的皮肤下进行皮下注射和后大腿进行肌肉注射。每个区域大约用1/4的免疫原。这样免疫原可以持久存在从而提高免疫应答。5. 取血：用19号针头对兔子进行耳动脉取血，室温过夜析出血清。6. 时间：第1星期 采血
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
coupled with mass spectrometry (AP?MS) is a robust technique used to identify protein?protein interactions. With recent improvements in sample preparation, and dramatic advances in MS instrumentation speed and sensitivity, this technique
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