- ￥1350 - 3000
- Proteintech
- 20960-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human HIF1a protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
HIF-1 alpha
- 抗体英文名：
HIF-1 alpha Polyclonal antibody
- 抗体名：
HIF-1 alpha Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Cobalt Chloride treated HeLa cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
Samples need to be treated with hypoxia stimulation.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20960-1-AP targets HIF-1 alpha in WB, IHC, IF/ICC, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig, chicken, goat |
| 免疫原 |
CatNo: Ag15198 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Domain: of Sequence: |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| 别名 | HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1 |
| 计算分子量 | 826 aa, 93 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC012527 |
| 基因名称 | HIF1A |
| Gene ID (NCBI) | 3091 |
| RRID | AB_10732601 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16665 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| WB | Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cellsAuthors - Decai Wang | |
| human | WB | Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.Authors - Ling Li |
| IF | Cancer Cell Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunityAuthors - Agnieszka Chryplewicz | |
Nat Cell Biol A single-cell transcriptomic landscape of the lungs of patients with COVID-19.Authors - Si Wang | ||
| WB | ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic RecyclingAuthors - Bide Tong | |
| human | WB | Mol Cell The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolismAuthors - Wang Jiahui |
accumulation by LPS, as TLR4 can use either MyD88 or TRIF. The net result of these interactions was activation of the transcription factors NF-{kappa}B and AP-1, and synthesis of TNF-{alpha} mRNA. TNF-{alpha} acts through an apparent autocrine pathway
Molecular Imaging of RNA Interference Therapy Targeting PHD2 for Treatment of Myocardial Ischemia
of hypoxia inducible factor-1 alpha (HIF-1α) transcriptional factor can activate several downstream angiogenic genes. However, HIF-1α is naturally degraded by prolyl hydroxylase-2 (PHD2) protein. Recently, we cloned the mouse PHD2 gene by comparing
我的试验动物是猪，在网上查的HIF的基因不是全序列，对引物设计有影响吧，如果这样不能得到引物我该怎样办？我急用求高人指点 LOCUS AY485675 569 bp mRNA linear MAM 23-DEC-2003 DEFINITION Sus scrofa hypoxia-inducible factor 1 alpha mRNA, partial cds. ACCESSION AY485675 VERSION AY485675.1 GI:40037189 KEYWORDS
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