- ￥1350 - 3000
- Proteintech
- 18251-1-AP
- 2025年10月13日
- WB, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PDCD1LG2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
PD-L2/CD273
- 抗体英文名：
PD-L2/CD273 Polyclonal antibody
- 抗体名：
PD-L2/CD273 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse heart tissue, HepG2 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18251-1-AP targets PD-L2/CD273 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag13039 Product name: Recombinant human PDCD1LG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-273 aa of BC074766 Sequence: MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPSCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | programmed cell death 1 ligand 2 |
| 别名 | B7 DC, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, PD-L2, PD-L2/CD273, Programmed death ligand 2 |
| 计算分子量 | 273 aa, 31 kDa |
| 观测分子量 | 40-50 kDa, 28 kDa |
| GenBank蛋白编号 | BC074766 |
| 基因名称 | PD-L2 |
| Gene ID (NCBI) | 80380 |
| RRID | AB_2878523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQ51 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IHC | Aging Cell Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.Authors - Qixia Xu |
| human | WB | J Nutr Biochem Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expressionAuthors - Qi Zhang |
| human | IHC | Cancer Sci ANO9 regulates PD-L2 expression and binding ability to PD-1 in gastric cancer.Authors - Keita Katsurahara |
| human | IHC | J Cell Mol Med IFI30 expression is an independent unfavourable prognostic factor in glioma.Authors - Xiu Liu |
| human | WB | J Transl Med Treatment with eFT-508 increases chemosensitivity in breast cancer cells by modulating the tumor microenvironment.Authors - Zhao-Ying Yang |
| human | IHC | Oncol Rep Differential expression of PD‑L1 and PD‑L2 is associated with the tumor microenvironment of TILs and M2 TAMs and tumor differentiation in non‑small cell lung cancer.Authors - Ryota Sumitomo |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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