- ￥1350 - 3000
- Proteintech
- 15361-1-AP
- 2025年10月13日
- WB, IP, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Arrestin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, IHC, ELISA
- 靶点：
Beta Arrestin 1
- 抗体英文名：
Beta Arrestin 1 Polyclonal antibody
- 抗体名：
Beta Arrestin 1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, U-937 cells, rat lung tissue, mouse lung tissue, RAW 264.7 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue, mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15361-1-AP targets Beta Arrestin 1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7608 Product name: Recombinant human Arrestin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 69-418 aa of BC003636 Sequence: DVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGKACGVDYEVKAFCAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYADICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTPFLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEEPPHREVPENETPVDTNLIELDTNDDDIVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | arrestin, beta 1 |
| 别名 | ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1, β-Arrestin-1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47-55 kDa |
| GenBank蛋白编号 | BC003636 |
| 基因名称 | Beta Arrestin 1 |
| Gene ID (NCBI) | 408 |
| RRID | AB_2060227 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49407 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).
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文献和实验| Species | Application | Title |
|---|---|---|
| human,mouse | WB,CoIP | Cell Metab Autonomous sensing of the insulin peptide by an olfactory G protein-coupled receptor modulates glucose metabolism.Authors - Jie Cheng
|
| mouse | IF | J Cell Biol BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.Authors - Fan Ye |
| IF | Cell Death Dis β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.Authors - J Liu | |
| human | WB | J Biol Chem An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.Authors - Jing-Yu Lin
|
| human | WB | PLoS One Cell Survival Following Radiation Exposure Requires miR-525-3p Mediated Suppression of ARRB1 and TXN1.Authors - Kraemer Anne A |
| mouse | IF | Oncol Rep Blue light irradiation inhibits the growth of colon cancer and activation of cancer‑associated fibroblasts.Authors - Toshiaki Yoshimoto |
10X TBS：24.2 g Trisbase, 80 g NaCl；用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液：1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T)：1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释：1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note： 一般来说，BSA被推荐用于多克隆抗体
10X TBS：24.2 g Trisbase, 80 g NaCl;用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液：1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T)：1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释：1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note： 一般来说，BSA被推荐用于多克隆抗体
7. 甲醇 8. TBS/T缓冲液：1X TBS, 0.1% Tween-20 9. 封闭缓冲液（TBS/T）：1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释：1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note： 一般来说，BSA被推荐用于多克隆抗体，脱脂奶粉用于单克隆抗体，这样可得到较高的信噪比。预染的蛋白质Marker，可用于监测转膜的效率。 样品制备 原始样品可为细胞、组织
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