- ￥1350 - 3000
- Proteintech
- 13883-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PRKCI protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
PKC Iota
- 抗体英文名：
PKC Iota Polyclonal antibody
- 抗体名：
PKC Iota Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13883-1-AP targets PKC Iota in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 |
CatNo: Ag4990 Product name: Recombinant human PRKCI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-587 aa of BC022016 Sequence: SSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSMSVKAASVLKSFLNKDPKERLGCLPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNERVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase C, iota |
| 别名 | PRKCI, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota, DXS1179E |
| 计算分子量 | 68 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC022016 |
| 基因名称 | PKC Iota |
| Gene ID (NCBI) | 5584 |
| RRID | AB_2171777 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41743 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF | Mol Cancer circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.Authors - Wei Gao
|
| chicken | IHC,IF | Development Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.Authors - Jianqiong Lin
|
| human,mouse | WB,IHC,IF,CoIP | Mol Med Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylationAuthors - Juying Jiao |
| human | WB | Int J Biol Sci Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.Authors - Fangfei Li |
| human | WB,IHC | Onco Targets Ther Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression.Authors - Liu Liu
|
| human | WB,IHC,IP | Antioxidants (Basel) PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4Authors - Hao Tao
|
列为5`-TGACTCA-3`）。TRE命名是因为TPA可通过活化PKC而诱导AP-1并与AP-1结合位点结合。 IL-2基因在转录起始部位的5`端含有一个增强子，它仅存在T细胞中以细胞特异性的方式控制IL-2基因的转录，几种结合到IL-2基因增强子区域的DNA结合蛋白被认为是MHC/Ag结合TCR与IL-2基因转录调节之间重要的联系环节。这个增强子中的一些序列可以特异性地同一个称为AP-1的转录激活蛋白相结合。AP-1可存在于不同细胞类型中，许多不同基因的调节区域
用于免疫检测的特殊配方的抗体稀释液，可以促进抗原抗体特异性结合，产生比传统方法高几倍或十几倍的信号强度，从而解决免疫检测实验中（比如免疫印迹，ELISA等）经常遇到的低灵敏度和高背景强度等问题，得到准确清晰的实验结果。 试剂盒操作简单方便，含有溶液1和2两种即用型溶液，分别用做一抗和二抗的稀释液，替代其他的传统稀释缓冲液，包括TBS，TBST，PBS，PBST等。其余操作均按照标准的免疫检测操作流程进行。此外，试剂盒中各组分对标记抗体的酶活性（辣根过氧化物酶HRP，碱性磷酸酶AP等）均没有影响
-AT主要参与T细胞的活化。上面已经提到，胞质中无活性状态的NF-AT以磷酸化的形式存在，称为NF-ATp，钙调磷酸酶作用后使其脱磷酸化而被激活并发生转位。而钙调磷酸酶能发挥作用，是信号转导的结果。 2．NF-κB NF含义同上。κB指B细胞x链，由两个亚单位p50和p65组成，其发现和定名是因为参与B细胞活化。现知它是一种分布广泛和十分重要的转录因子。通常，NF-κB在胞质中和抑制因子LxB以复合物的形式存在。PKC可使I-κB发生磷酸化造成该复合物解离，从而解除了I-κB对NF-κB
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